Instruments in the Omics Hub

Discover the instruments that are available for research needs in the Omics Hub.

Agilent Bioanalyzer 2100

Instrument for quality control, sizing, and quantification of DNA, RNA, and proteins. Example kits include:

• DNA 1000 kit
• High sensitivity kit
• RCA 6000 nano kit
• RCA 6000 pico kit

Autoclave

Steris SV-120 steam autoclave for sterilization of laboratory supplies. Please do not use for biowaste.

Beckman Coulter Avanti J-E Centrifuge

Compact, high-performance refrigerated centrifuge with max speed of 21,000 RPM or 53,300 x g. Multiple rotors available for centrifuging larger containers (15mL, 50 mL, and 250 mL).

Bioinformatics Station

We have two PC’s available for Next Generation Sequence analysis:

1. Dell Precision 7920 Tower (128 GB RAM, 2 TB x 7 disk space) for CLC Genomics use
2. Dell 7600 (250 GB RAM, 4 TB disk space) for Sequencher use

We have various other software available for Omics analysis:

• Image Quant
• Milliplex Analyst
• Primer Express
• LCsolution

BioRad Gradient Thermocycler

BioRad Mycyler for PCR, including gradient PCR capability.

Biotek Synergy H1 Microplate Reader

The Synergy H1 Microplate Reader in Room 332 is for fluorescence, absorbance and luminescence measurements and was designed with an emphasis on superior performance in all detection methods.

• Automatic top and bottom epifluorescence read modes optimized for both sensitive homogeneous and cell-based assays
• Compatible with Take3 Multi-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
• Software-controlled automatic probe height adjustment with built-in plate sensor to prevent probe crashes
• Scanning monochromator for wavelength scans to identify peak absorbance and provide full spectral analysis
• Luminescence measurements with top detection for best sensitivity
• Reads 6- to 384-well microplate formats including PCR trays and Terasaki plates
• Well area scanning for popular cell-based assays
• Time-Resolved Fluorescence capability

Learn more about the Biotek Synergy H1 Microplate Reader

Cellometer Vision

An automated cell counter and analyzer that combines brightfield and multi-channel fluorescence to generate cell count & florescence data.

Explore the Cellometer Vision

EVOS M7000 Imaging System

Designed for advanced research applications, the EVOS M7000 delivers automated, multi-channel fluorescence and transmitted light imaging in an inverted system configuration.

Objectives: 2X, 4X, 10X, 20X(Phase, 0.40 NA / 3.1 WD), 40X(Phase, 0.75 NA / 0.72 WD), 100X (Oil – 1.28 NA / 0.21 WD)

EVOS LED Cubes: DAPI, GFP, RFP, Texas Red, and Cy5

Capabilities: Automated time-lapsed imaging, Area scanning, Tile stitching, and Automated stage

Discover the EVOS M7000 Imaging System from ThermoFisher Scientific’s website.

Focused – Ultrasonicator

Covaris M220 for DNA shearing via mechanical shearing of nucleic acids by ultrasonic waves.

FreeZone Freeze Dryer

LabConCo FreeZone Stoppering Tray Dryer to lyophilize samples and stopper them under vacuum, and FreeZone 12 Liter -84° C Console Freeze Dryer.

Luminex 200

Multiplexing immunoassay system with features including:

• Beads labeled with varying ratios of two fluorescent dyes, and can resolve 100 different dye sets
• Classify and quantify up to 100 analytes / well in a micotiter plate.
• Analyze from one SNP in one individual up to 50 SNPs in unlimited numbers of individuals
• Liquid reaction kinetics enables faster and more reproducible results
• It’s open architecture platform allows leading companies to provide compatible kits and software.

This system allows multiplexing of up to 100 analytes in a single well of a microtiter plate. It’s open architecture platform allows leading companies to provide compatible kits and software. The Milliplex Analyst software makes data analysis very easy.

Read more on the Luminex 200 on Diasorin’s website.

MACSQuant Analyzer 10

Flow cytometer designed for simple, automated, and hands-free operation. Features of this equipment:

• 3 lasers, 10 optical channels
• Multi sample analysis of up to 96 wells
• Integrated auto-labeling

Milli-Q Advantage A-10 Water Purification System

Purifies pre-treated pure water into Type 1 ultrapure water to use with critical workflows.

Water Specifications:

• Resistivity 18.2 MΩcm at 25°ree;C
• TOC ≤ 5 ppb
• Particulates* (> 0.22 µm/ml) < 1 particulate/ml
• Bacteria* < 1 cfu/ml
• Pyrogens (endotoxins)* < 0.001 EU/ml
• RNases* < 0.01 ng/ml
• DNases* < 4 pg/µl
• Flow Rate up to 2 l/min

*Test conditions with the appropriate Q-POD final polisher. These values are typical and may vary depending on the nature and concentration of contaminants in the feed water.

Nanodrop One C

Spectrophotometer that quantifies DNA, RNA, and proteins in 1–2 μ L samples.

Features of this equipment:

• full-spectrum UV-Vis absorbance analyses (220-750nm)
• for measuring absorbance of DNA, RNA, dyes, proteins and microbial cell culture OD
• One µl of sample
• Large dynamic range: 2-3700 ng/µl of dsDNA
• No dilutions for most samples
• Cuvette position available
• Ten second measurement

Qiagen TissueLyser II

Tissue homogenizer that rapidly disrupts and lyses biological samples.

This instrument is equipped with:

• Grinding jar Set
• Adapter set 2 x 24
• Adapter set 2 x 96

QuantStudio Digital Droplet PCR

Droplet Digital PCR (ddPCR) utilizes Taq polymerase in a standard PCR reaction to amplify target. DNA fragment from a complex sample using pre-validated primer or primer/probe assays. This technology distributes the PCR reaction into 20,000 wells and acquires data at the end of the reaction. These factors offer the advantage of direct and independent quantification of DNA without standard curves. In addition, end-point measurement enables nucleic acid quantitation independently of the reaction efficiency, resulting in a positive-negative call for every droplet and greater amenability. DDPCR technology can be used for extremely low-target quantitation.

Learn more about the QuantStudio Digital Droplet PCR from ThermoFisher Scientific’s website.

QuantStudio6 Flex qPCR

Real-time PCR instrument used for Quantitative study of gene expression, genotyping, SNP analysis, pathogen detection and measuring RNA interference.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate.
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.
• 96- or 384-well plate compatibility — including the TaqMan® Low Density Array.
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security.
• Proven assay development guidelines save time and money

Discover the QuantStudio6 Flex qPCR from ThermoFisher Scientific’s website.

Qubit 2.0 Fluorometer

Fluorescence based quantification of DNA, RNA, and proteins.

How does Qubit work? Who should use Qubit?

• full-spectrum UV-Vis absorbance analyses (220-750nm)
• for measuring absorbance of DNA, RNA and proteins OD and quantitative assays.
• Large LCD color touch screen
• Easy workflow navigation to save time
• Automatic data logging and USB port
• Standard curve display after calibration is complete

Learn more about the Qubit 2.0 Fluorometer from ThermoFisher Scientific’s website.